A new membrane based process to isolate immunoglobulin from chicken egg yolk
نویسندگان
چکیده
منابع مشابه
identification and extraction of chicken egg yolk immunoglobulin from egg by polyethylene glycol (peg) precipitation
background : staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. treatment of patients with these infections has been difficult. the aim of this study was to detect and extract, the egg yolk immunoglobulin y as a potential source of anti- s. aureus antibody. methods : specific igy was produced by immunizing hens wi...
متن کاملSequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents.
A study was conducted to develop a simple sequential separation protocol to separate phosvitin and IgY from egg yolk without using organic solvents. Egg yolk was diluted with 2 volumes of distilled water (DW), homogenized, and centrifuged. The precipitant was collected and homogenized with 4 volumes of 10% NaCl (wt/vol) in 0.05 N NaOH solution to extract phosvitin. The pH of the homogenate was ...
متن کاملThe potency of chicken egg yolk immunoglobulin (IgY) specific as immunotherapy to Mycobacterium tuberculosis infection
The aim of this study was to characterize of chicken egg yolk immunoglobulins (IgYs) specific as immunotherapy to Mycobacterium tuberculosis complex (MTBC) infection. Lohmann laying hens were immunized intramuscularly with antigenic of MTBC. Egg yolk was separated from egg white, and IgY antibody was then purified by multiple polyethylene glycols 6000 extraction and ammonium sulfate purificatio...
متن کاملA simple method for isolating chicken egg yolk immunoglobulin using effective delipidation solution and ammonium sulfate.
Chicken egg yolk immunoglobulin (IgY) is a superior alternative to mammalian immunoglobulin. However, the practical application of IgY in research, diagnostics, and functional food is limited due to complex or time-consuming purification procedures. The objective of this study was to develop a simple, safe, large-scale separation method for IgY from egg yolk. Egg yolk was diluted with 6-fold de...
متن کاملOccurrence of glycosphingolipids in chicken egg yolk.
Chicken egg yolk was found to contain a unique glycosphingolipid pattern not seen in other types of tissue or cell. These glycosphingolipids were isolated in pure form and their structures established by sequential enzymic hydrolysis and permethylation analysis. The major gangliosides in chicken egg yolk are N-acetylneuraminosylgalactosylceramide, N-acetylneuraminosyl-lactosylceramide and di-N-...
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ژورنال
عنوان ژورنال: Food Chemistry
سال: 2010
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2010.03.048